COURSE OVERVIEW
Who is this course for?
This course is relevant to anyone using static headspace sampling for GC analysis. It is applicable to most instrument designs.
Previous Knowledge
Good knowledge of chromatography and associated data.
What you will learn
- General concepts and benefits
- Most critical parameters for HS sampling
- GC requirements and specifics of HS instrumentation
- Guidelines to method development
- Quantitative approaches to HS analysis
COURSE OUTLINE
Fundamentals of Headspace GC
- Principles of Headspace Analysis
- Boyle’s Law and Dalton’s Law of Partial Pressures
- Understanding Matrix Effects
Instrument Design and Sample Introduction
- Vial Pressurization Methods
- Sampling Valve and Carrier Flow Control
- Loop Filling and Transfer Line Considerations
- Common Headspace GC Problems and Troubleshooting
Headspace Sample Preparation
- Selection of Headspace Vial Types
- Closure Types and Their Effect on Sample Integrity
- Evaluating Closure Quality and Pressure Integrity
Internal Standards and Calibration Strategies
- Choosing an Appropriate Internal Standard
- Optimizing Internal Standard Performance
GC Instrumentation Requirements for Headspace Analysis
- Selecting the Right Inlet System
- Carrier Gas and Flow Requirements
- Optimizing Liner Types and Oven Conditions
Headspace Method Development and Optimization
- Solvent Selection and Optimization
- Effect of Oven Conditions on Headspace Extraction
- Fill Volume Considerations and Salting-Out Effect
- Sampling Conditions for Maximum Sensitivity
Advanced Headspace Techniques
- Total Headspace Extraction (THS)
- Multiple Headspace Extraction (MHE)
- Standard Addition Method for Quantification
Q&A and Closing Discussion
- Summary of Key Concepts
- Open Discussion on Challenges Faced in Headspace Analysis
- Resources for Further Learning